Research Paper Volume 16, Issue 17 pp 12225—12238
Melatonin downregulates angiogenesis and lymphangiogenesis by regulating tumor-associated macrophages via NLRP3 inflammasomes in lung adenocarcinoma
Figure 7. Melatonin downregulated angiogenesis and lymphangiogenesis by targeting the NLRP3 pathway in mice. (A) Levels of angiogenic and lymphangiogenic factors (VEGF-A, VEGF-B, VEGF-C, CXCL5, and CXCL8) in mice serum determined by ELISA. (B–E) Immunohistochemical (IHC) staining of vascular marker proteins (CD34, CD31, and vWF) in tumor tissues. (F, G) Immunofluorescence (IF) detection of VEGF in tumor tissues. (H–J) IF detection of neovascularization marker (CD31) and lymphangiogenesis marker (LYVE-1) in tumor tissues. *p < 0.05, **p < 0.01, vs. control group. Data provided as mean ± SD (n = 3).