Research Paper Volume 16, Issue 17 pp 12225—12238
Melatonin downregulates angiogenesis and lymphangiogenesis by regulating tumor-associated macrophages via NLRP3 inflammasomes in lung adenocarcinoma
Figure 3. The NLRP3 inflammasomal axis positively modulated angiogenesis and lymphangiogenesis. (A) The NLRP3, ASC, Caspase-1, IL-1β, and IL-18 transcript expressions in the control, si-NLRP3, and oe-NLRP3 cells, as evidenced by RT-PCR. (B) The NLRP3, ASC, Caspase-1, IL-1β, and IL-18 protein contents in the control, si-NLRP3, and oe-NLRP3 cells, as evidenced by western blot assay. (C) The VEGF-A, VEGF-B, VEGF-C, CXCL8, and CXCL5 protein expressions in the control, si-NLRP3, and oe-NLRP3 cells, as detected by ELISA. (D) The VEGF-A, VEGF-B, VEGF-C, CXCL8, and CXCL5 protein contents, as detected by ELISA in control, IL-1β Ab-, and IL-18 Ab-treated cells. (E, F) Branch point quantification. Typical phase-contrast images revealing tube formation. *p < 0.05, **p < 0.01. Data provided as mean ± SD (n = 3).