Research Paper Volume 16, Issue 17 pp 12225—12238
Melatonin downregulates angiogenesis and lymphangiogenesis by regulating tumor-associated macrophages via NLRP3 inflammasomes in lung adenocarcinoma
Figure 1. Co-culturing THP-1 macrophages with A549 strongly enhanced the NLRP3 inflammasomal axis expressions in A549. (A) The NLRP3, ASC, Caspase-1, IL-1β, and IL-18 transcript contents were substantially augmented in the A549 and THP-1 co-culture system. (B, C) The NLRP3, ASC, Caspase-1, IL-1β, and IL-18 protein expressions were upregulated in the A549 and THP-1 co-culture system, as evidenced by western blot assay. β-actin served as the endogenous control. *p < 0.05, **p < 0.01. Data provided as mean ± SD (n = 3).