Research Paper Volume 16, Issue 16 pp 11857—11876
Circ_0006476 modulates macrophage apoptosis through the miR-3074-5p/DLL4 axis: implications for Notch signalling pathway regulation in cardiovascular disease
Figure 7. Circ_0006476/miR-3074-5p sponge adsorption function. (A) qPCR analysis of DEmiRNA expression levels. (B) Volcano plot displaying miR-3074-5p expression. (C) The predicted binding sites of DLL4 and circ_0006476 on miR-3074-5p. (D) qPCR assessment of the impact of miR-3074-5p knockdown on miR-3074-5p and apoptosis-related genes. (E) WB analysis showing the effects of miR-3074-5p knockdown on apoptosis and Notch signalling pathway-related proteins. (F) Hoechst staining of THP-1 macrophages (bright blue represents the apoptotic cell nucleus) (scale bar: 50 μm). (G) Verification of the sponge adsorption relationship between circ_0006476 and miR-3074-5p. (n = 3. The data are presented as the mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the control group; #P < 0.05 and ##P < 0.01 vs. the nicotine group).