Research Paper Volume 16, Issue 21 pp 13304—13322
Angiotensin-(1-7) relieves behavioral defects and α-synuclein expression through NEAT1/miR-153-3p axis in Parkinson’s disease
Figure 5. Ang-(1-7) decreases the level of α-syn and apoptosis in the hα-syn(A53T) overexpressed dopaminergic neurons through NEAT1. (A) The NEAT1 level in each group of mice was tested by qRT-PCR (n = 3). (B) The α-syn level of primary dopaminergic neurons in each group was shown by Western blot (n = 3). (C) Quantitative evaluation of α-syn level. (D) Cell cytotoxicity of primary dopaminergic neurons in each group was assessed by LDH assay (n = 3). (E) The levels of Bcl2, Bax and cleaved caspase-3 in every group were analyzed by Western blot (n = 3). (F) Quantitative evaluation of Bcl2 level. (G) Quantitative evaluation of Bax level. (H) Quantitative evaluation of Cleaved caspase-3 level. Data are shown as the mean ± SD. **P<0.01 versus hα-syn(A53T) or hα-syn(A53T) + LV-NC group; ****P<0.0001 versus hα-syn(A53T) + LV-NC group; #P<0.05, ###P<0.001 and ####P<0.0001 versus the hα-syn(A53T) + Ang-(1-7) + LV-NC group.