Research Paper Volume 16, Issue 21 pp 13304—13322
Angiotensin-(1-7) relieves behavioral defects and α-synuclein expression through NEAT1/miR-153-3p axis in Parkinson’s disease
Figure 4. NEAT1 acts as a sponge of miR-153-3p therefore imcreasing the level of α-syn and apoptosis in the hα-syn(A53T) overexpressed dopaminergic neurons. (I) Cell cytotoxicity of primary dopaminergic neurons in each group was tested by LDH assay (n = 3). (J) The levels of Bcl2, Bax and cleaved caspase-3 in every group were observed by Western blot (n = 3). (K) Quantitative evaluation of Bcl2 level. (L) Quantitative evaluation of Bax level. (M) Quantitative evaluation of Cleaved caspase-3 level. Data are shown as the mean ± SD. *P<0.05 and ****P<0.0001 versus the hα-syn(A53T) + LV-NC group; **P<0.01 versus the NC mimic or hα-syn(A53T) + LV-NC group; ***P<0.001 versus the LV-NC or hα-syn(A53T) + LV-NC group; #P<0.05, ##P<0.01 and ####P<0.0001 versus hα-syn(A53T) + sh-NC group; ###P<0.001 versus the sh-NC group.