Research Paper Volume 16, Issue 21 pp 13304—13322

Figure 4. NEAT1 acts as a sponge of miR-153-3p therefore increasing the level of α-syn and apoptosis in the hα-syn(A53T) overexpressed dopaminergic neurons. (A, B) Probable binding site of NEAT1 to miR-153-3p. (C) The dual luciferase reported assay to verify the combination between NEAT1 and miR-153-3p (n = 3). (D) The location of NEAT1(red) and miR-153-3p(green) in the brain section of mice. TH (pink) represents the location of dopaminergic neurons, whereas DAPI (blue) represents the location of nuclear localization. Scale bar, 50 μm (n = 6). (E) The NEAT1 levels of primary dopaminergic neurons in indicated groups were analysed by qRT-PCR (n = 3). (F) The miR-153-3p levels of primary dopaminergic neurons in indicated groups were assessed by qRT-PCR (n = 3). (G) The α-syn level of primary dopaminergic neurons in each group was detected by Western blot (n = 3). (H) Quantitative evaluation of α-syn level.