Research Paper Volume 16, Issue 21 pp 13304—13322
Angiotensin-(1-7) relieves behavioral defects and α-synuclein expression through NEAT1/miR-153-3p axis in Parkinson’s disease
Figure 3. Ang-(1-7) decreases the level of α-syn and apoptosis in the hα-syn(A53T) overexpressed dopaminergic neurons through miR-153-3p. (A) The α-syn level of primary dopaminergic neurons in each group was detected by Western blot (n = 3). (B) Quantitative evaluation of α-syn level. (C) Cell cytotoxicity of primary dopaminergic neurons in each group was tested by LDH assay (n = 3). (D) The levels of Bcl2, Bax and cleaved caspase-3 in every group were observed by Western blot (n = 3). (E) Quantitative evaluation of Bcl2 level. (F) Quantitative evaluation of Bax level. (G) Quantitative evaluation of Cleaved caspase-3 level. Data are shown as the mean ± SD. **P<0.01 and ***P<0.001 versus the hα-syn(A53T) + NC inhibitor group; #P<0.05, ##P <0.01 and ###P<0.001 versus the hα-syn(A53T) + Ang-(1-7) + NC inhibitor group.