Research Paper Volume 16, Issue 21 pp 13304—13322

Figure 2. Overexpression of miR-153-3p reduces the level of α-syn and relieves the apoptosis in the hα-syn(A53T) overexpressed dopaminergic neurons. (A) The heatmap showed differentially expressed miRNAs in right SN of mice for every group (n = 3). (B) Probable binding site of miR-153-3p to α-syn. (C) The luciferase reporter assay to verify the combination between miR-153-3p and α-syn (n = 3). (D) The miR-153-3p level in SN of each mice group were assessed by qRT-PCR (n = 3). (E) The miR-153-3p levels of primary dopaminergic neurons in indicated groups were analysed by qRT-PCR (n = 3). (F) The α-syn level of primary dopaminergic neurons in each group was tested by Western blot (n = 3). (G) Quantitative evaluation of α-syn level.