Research Paper Volume 16, Issue 15 pp 11568—11576
Circular RNA circMRPS35 represses malignant progression in osteosarcoma cells via targeting miR-105-5p/FOXO1
Figure 3. CircMRPS35 serves as a sponge of miR-105-5p to promote FOXO1 expression in osteosarcoma cells. (A) The interaction of circMRPS35 and miR-105-5p was predicted in ENCORI database. (B) The expression of miR-105-5p was measured by qPCR in MG63 and MNNG/HOS cells treated with circMRPS35 overexpressing plasmid. (C, D) The MG63 and MNNG/HOS cells were transfected with miR-105-5p mimic. (C) The expression of miR-105-5p was measured by qPCR. (D) The luciferase activity of circMRPS35 was analyzed by luciferase reporter gene assay. (E) The interaction of FOXO1 and miR-105-5p was predicted in ENCORI database. (F–H) The MG63 and MNNG/HOS cells were transfected with miR-105-5p mimic. (F) The expression of miR-105-5p was measured by qPCR. (G) The expression of FOXO1 was detected by qPCR. (H) The luciferase activity of FOXO1 3’UTR was analyzed by luciferase reporter gene assay. (I) The expression of FOXO1 was detected by Western blot analysis in MG63 and MNNG/HOS cells treated with circMRPS35 overexpressing plasmid and miR-105-5p mimic. **P < 0.01.