Research Paper Volume 16, Issue 19 pp 12806—12819
FGA influences invasion and metastasis of hepatocellular carcinoma through the PI3K/AKT pathway
Figure 5. FGA inhibits the epithelial-mesenchymal transition (EMT) through the PI3K/AKT signaling pathway. (A) Western blot analysis of the expression levels of key EMT proteins (E-cadherin, N-cadherin, slug) in the control group, FGA knockout group and FGA overexpressed group, and their quantitative analysis (B). (C) Immunofluorescence staining of the expression levels of E-cadherin, N-cadherin, and vimentin proteins in the control group, FGA knockout group and FGA overexpressed group, and their quantitative analysis (D). (E, F) Western blot analysis of the expression levels of proteins related to the PI3K/AKT signaling pathway in the control group, FGA knockout group and FGA overexpressed group, and their quantitative analysis. (mean ± SD, n = 3; *P < 0.05, **P < 0.01, ***P < 0.001, compared with the control group. Scale: 20 μm)