Research Paper Volume 16, Issue 13 pp 11103—11116

Figure 2. HOXC4 regulates PC cell proliferation, apoptosis and cell cycle distribution in vitro. (A, B) The transfection efficiency of HOXC4 overexpression lentivirus (HOXC4-OE) and HOXC4 shRNAs (sh-HOXC4). (C) Cell counting kit-8 assay was used to detect the effect of HOXC4 on PC cell proliferation. (D) Colony formation assay was used to detect the effect of HOXC4 on PC cell colony formation. (E, F) Flow cytometry was used to detect the effect of HOXC4 on PC cell apoptosis. (G, H) Flow cytometry was used to detect the effect of HOXC4 on the cell cycle distribution of PC cells. (I) Western blotting was used to detect the expression of Bcl-2, Bax, caspase 3, cleaved caspases 3, cyclin-dependent kinase 1 and cyclin D1 while HOXC4 was overexpressed and inhibited. ^**P < 0.01.