Research Paper Advance Articles

Figure 3. Overexpression of AK7 inhibited proliferation, invasion and migration of human ccRCC cell lines. (A, B) qRT-PCR verified the efficiency of overexpression of AK7 in 786-O and AKI-1 cell lines. (C, D) The growth curves of 786-O (C) and Caki-1 (D) cells were plotted after overexpression of AK7 based on CCK-8 assay. (E, F) Colony formation assays demonstrated that overexpression of AK7 inhibited the proliferation of 786-O and Caki-1 cells. (G–I) Transwells experiment demonstrated that overexpression of AK7 could effectively inhibit the migration and invasion ability of ccRCC cells. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ^****P < 0.0001.