Research Paper Advance Articles

Figure 2. AK7 knockdown promoted the proliferation, invasion and migration ability of human ccRCC cell lines. (A, B) Three siRNAs (si1, si2, and si3) were designed to silence AK7 in ccRCC cells (786-O and Caki-1), and validated by qRT-PCR. (C, D) The growth curves of 786-O (C) and Caki-1 (D) cells were plotted after transfection with si1-AK7/si-NC based on CCK-8 assay. (E, F) Colony formation assays demonstrated that knockdown of AK7 promoted the proliferation of 786-O and Caki-1 cells. (G–I) Transwells experiment demonstrated that knockdown of AK7 expression could effectively promote the migration and invasion ability of ccRCC cells. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ^****P < 0.0001.