Research Paper Volume 16, Issue 18 pp 12510—12524

Figure 6. Effects of DANCR overexpression on the expression of membrane cholesterol transporters in ox-LDL-treated THP-1 macrophages. THP-1 monocytes were incubated with 160 ng/mL PMA for 48 h to differentiate into macrophages. Then, they were treated with 50 μg/mL ox-LDL for 24 h, followed by transfection with 1×10¹⁰ TU/mL LV-NC or LV-DANCR 48 h. (A–E) Detection of the mRNA levels of ABCA1, ABCG1, SR-BI, SR-A, and CD36 by qPCR. (F–K) Detection of the protein levels of ABCA1, ABCG1, SR-BI, SR-A, and CD36 by Western blot. All results are presented as the mean ± SD from three independent experiments performed in triplicate. n=3.