Figure 5.Effects of DANCR inhibition on the expression of membrane cholesterol transporters in ox-LDL-treated THP-1 macrophages. THP-1 macrophages were treated with 50 μg/mL ox-LDL for 24 h. Then, they were transfected with 1×1010 TU/mL LV-DANCR or 20 μM si-DANCR or their corresponding control for 48 h. (A–E) Detection of the mRNA levels of ABCA1, ABCG1, SR-BI, SR-A, and CD36 by qPCR. (F–K) Detection of the protein levels of ABCA1, ABCG1, SR-BI, SR-A, and CD36 by Western blot. All results are presented as the mean ± SD from three independent experiments performed in triplicate. n=3.
