Research Paper Volume 16, Issue 18 pp 12510—12524

Figure 4. DANCR promotes lipid uptake and inhibits cholesterol efflux in THP-1 macrophages. THP-1 macrophages were treated with 50 μg/mL ox-LDL for 24 h. Then, they were transfected with 1×10¹⁰ TU/mL LV-DANCR or 20 μM si-DANCR or their corresponding control for 48 h. (A, B) Cells were incubated with 10 μg/μL Dil-ox-LDL at 37° C for 4 h. The representative fluorescent images of Dil-ox-LDL uptake were photographed (200×). (C, D) Cells were loaded with 5 μM NBD-cholesterol for 6 h. Then, the medium was supplemented with 25 μg/mL apoA-I or 50 μg/mL HDL for 4 h. The representative fluorescent images of the NBD cholesterol burden in THP-1 macrophage-derived foam cells were photographed (200×). All results are presented as the mean ± SD from three independent experiments performed in triplicate. Scale bar=50 μm.