Research Paper Volume 16, Issue 18 pp 12510—12524

Figure 3. DANCR promotes lipid accumulation in THP-1 macrophages. (A) THP-1 macrophages were transfected with 20 μM si-DANCR or the negative control for 48 h. Transfection efficiency was determined by qPCR. (B) THP-1 macrophages were treated with 50 μg/mL ox-LDL for 24 h. Then, they were transfected with 20 μM si-DANCR for 24 h, 36 h, and 48 h, respectively. Oil Red O staining was used to detect lipid droplets. Image-Pro Plus software calculated Oil Red O-positive areas. (C) THP-1 macrophages were transfected with 1×10¹⁰ TU/mL LV-DANCR or the negative control for 48 h. Transfection efficiency was determined by qPCR. (D) THP-1 macrophages were treated with 50 μg/mL ox-LDL for 24 h. Then, they were transfected with 1×10¹⁰ TU/mL LV-DANCR for 24 h, 36 h, and 48 h, respectively. Oil Red O staining was used to detect lipid droplets. Image-Pro Plus software calculated Oil Red O-positive areas. All results are presented as the mean ± SD from three independent experiments performed in triplicate. Scale bar=200 μm.