Research Paper Volume 16, Issue 13 pp 10972—10984
Unveiling diabetic nephropathy: a novel diagnostic model through single-cell sequencing and co-expression analysis
Figure 1. Single-cell sequencing analysis of diabetic nephropathy (DN) and control samples. (A, B) Quality control metrics and sample integration results showing no significant batch effects between DN and control samples, with observed heterogeneity. (C–E) UMAP plots illustrating cell clustering into 16 distinct clusters based on cell type marker genes, with 10 cell types annotated. (F, G) Distribution of cell types across samples and the top 5 significantly up-regulated and down-regulated differential genes for each cell type in DN and control groups. (H, I) Enrichment fraction of disulfidptosis in Glomerular Cells and Connecting Tubule cells, with samples categorized into Disulifidptosis_High and Disulifidptosis_Low groups based on median values, highlighting differentially expressed genes between the two groups.