Research Paper Volume 16, Issue 13 pp 10670—10693
Roles of pigment epithelium-derived factor in exercise-induced suppression of senescence and its impact on lung pathology in mice
Figure 4. PEDF suppresses cellular senescence in lung tissues. A recombinant PEDF protein (10 μg/kg body weight) was intraperitoneally injected twice a week for 4 weeks. (A) The expression of Ink4a, Arf, and p21 in lung total RNA was analyzed by real-time PCR. Values were normalized to Gapdh in each sample. (B) Lung tissues were stained for SA-β-gal. Arrowheads indicate SA-β-gal-positive cells. Scale bar, 50 μm. (C) The number of SA-β-gal-positive cells was counted in each sample. Values represent means ± SEM. Data were analyzed by the Student’s t-test. *P <0.05 and **P <0.01.