Figure 7. Effects of ASP on apoptosis proteins in vivo. (A, B) Markers of apoptosis and the p38/MAPK signaling pathway were tested using Western blotting. (C, D) Markers of mitochondrial apoptosis and the p38/MAPK signaling pathway were tested using IHC (×400). The results for the treated group indicate that the medium-dose ASP treatment reduces mitochondrial apoptosis in BMNC cells. Note: *P < 0.05 and **P < 0.01 compared with the normal control group. ▲P < 0.05 and ▲▲P < 0.01 compared with the model control group.