Figure 5. Effects of AS on apoptosis in BMMC cells. (A) Immunofluorescence images of ROS. (B) The ROS levels of each group were assessed. (C) Flow cytometry scatter plot of apoptosis. (D) Apoptosis was assessed in different groups. (E, F) Markers of apoptosis were tested using Western blotting. (G) The colocalization of Bax with mitochondria (yellow dots). (H) Mitochondrial structure was detected by electron microscopy. Note: *P < 0.05 and **P < 0.01 compared with the normal control group. ▲P < 0.05 and ▲▲P < 0.01 compared with the model control group, #P < 0.05 and ##P < 0.01 compared with the low-dose group.