Research Paper Volume 16, Issue 15 pp 11513—11534
S100A9, as a potential predictor of prognosis and immunotherapy response for GBM, promotes the malignant progression of GBM cells and migration of M2 macrophages
Figure 5. Upregulation of S100A9 promoted the proliferation of GBM cells. (A) Differential expression of S100A9 protein between human astrocyte cells (NHA) and five GBM cell lines. (B, C) Transwell assay (B) and wound healing assay (C) were utilized to detect the migration ability of U87 and LN229 cells transfected with knockout and overexpression S100A9 lentivirus. (D–F) Colony formation assay (D), CCK-8 assay (E), and EdU staining (F) were utilized to detect the proliferative activity of U87 and LN229 cells transfected with knockout and overexpression S100A9 lentivirus.