Research Paper Volume 16, Issue 15 pp 11513—11534
S100A9, as a potential predictor of prognosis and immunotherapy response for GBM, promotes the malignant progression of GBM cells and migration of M2 macrophages
Figure 10. S100A9 is up-regulated in GBM tissues and contributes to the migration of M2 macrophages. (A, B) Immunohistochemical staining of iNOS (A) and CD206 (B) in GBM and its adjacent tissues. (C) Representative image of multiplex immunofluorescence staining of DAPI, iNOS, and S100A9 in GBM tissue. (D) Representative image of multiplex immunofluorescence staining of DAPI, CD206, and S100A9 in GBM tissue. (E) The process of inducing THP-1 cells into M2 macrophages. (F) Sketch map of co-culture of M2 macrophages and U87 cells transfected with knockout S100A9 lentivirus. (G, H) Transwell assay was utilized to detect the migration ability of M2 macrophages after co-culture with U87 cells transfected with lentivirus knockout S100A9 and LN229 cells transfected with lentivirus overexpressing S100A9.