Research Paper Volume 16, Issue 21 pp 13288—13303

Figure 8. (A) Sequences of mmu-miR-150-5p and CXCL12-wild type (CXCL12^WT) and CXCL12-mutant type (CXCL12^MUT). (B) Dual luciferase assay was used to detect the activity of CXCL12 wild type (CXCL^WT), CXCL12 mutant (CXCL^MUT), and mir-150-5p overexpression vector (mir-150-5p mimic) after transfection into primary microglia of mice. (C) qRT-PCR was used to detect the expression of mir-150-5p and CXCL12 in mouse primary microglia after transfection of miR-NC and miR-150-5p. (D) Western blot was used to detect the protein expression of CXCL12 in primary microglia of mice after transfection of miR-NC and miR-150-5p. Values were expressed as means ± SEM, n=3, *** P < 0.001.