Research Paper Volume 16, Issue 21 pp 13288—13303
Piperine attenuates cancer-associated pain induced by microglial activation via increasing miR-150-50p
Figure 5. (A) qRT-PCR detected the expression of miR-150-50p in the spinal cord of all mice. (B) FISH detected the miR-150-50p expression in the spinal cord of mice. (C) qRT-PCR detected the CXCL12 expression in the spinal cord of mice. (D) Western blot detected CXCL12 protein expression in the spinal cord of mice. (E) Immunofluorescence detected CXCL12 protein expression in the spinal cord of mice. Values were expressed as means ± SEM, n=8, ** P<0.01, *** P<0.001.