Research Paper Volume 16, Issue 21 pp 13288—13303

Piperine attenuates cancer-associated pain induced by microglial activation via increasing miR-150-50p

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Figure 3. (A) qRT-PCR detected the mRNA expression of SHISA4, TADA1, GDI1, HILPDA, CXCL12, and ABCB9 in the spinal cord of mice at different periods (0, 7, 14, and 21 days). (B) Western Blot detected the protein expression of CXCL12 in the spinal cord of mice at different periods. (C) Immunofluorescence was used to detect the protein expression of CXCL12 in the spinal cord of mice at different periods (0, 7, 14, and 21 days). Values were expressed as means ± SEM, n=8, * P<0.05, *** P<0.001.