Research Paper Volume 16, Issue 15 pp 11501—11512

Figure 4. AL161431.1 promotes CCA cell proliferation, migration and invasion in vitro. (A, B) Expression levels of AL161431.1 in QBC-939 and RBE cells treated with three siRNA. (C–L). Cell proliferation detection of QBC-939 and RBE cells were measured by CCK-8, EDU assay and clone formation (magnification, x100, scale bar, 100 um). (M–V). Cell migratory and invasive capabilities were assessed by wound healing assays and transwell assays in QBC-939 and RBE cells transfected with NC, si- AL161431.1-1 and si- AL161431.1-2 (magnification, ×100, scale bar, 100 μm). ^nsp ≥ 0.05, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.