Research Paper Volume 16, Issue 9 pp 7535—7552
Caloric restriction mitigates age-associated senescence characteristics in subcutaneous adipose tissue-derived stem cells
Figure 5. Effect of age on senescence in ASCs. (A) Representative histogram of senescence marker C12FDG fluorescence among ASCs from young and aged mice. (B, C) The mean fluorescence intensity distribution of C12FDG in subcutaneous (B) and visceral (C) ASCs from young and aged mice. Graphs are representative of eight mice per group and ASCs from two mice were pooled together to achieve 5000 events count. (D) The mean fluorescence intensity distribution of C12FDG in subcutaneous ASCs from mice fed on caloric restricted and ad-libitum diet. (n= 3–5 mice per group) P value < 0.05 = *, <0.00005 = ****, ns = non-significant.