Figure 1. Effect of age on body fat distribution. (A, B) Subcutaneous adipose tissue was surgically excised from male (A) and female (B) mice and weighed (n = 5). (C, D) Subcutaneous fat and total body mass were measured and the ratio was calculated and plotted (n = 5). (E, F) Visceral fat was surgically excised from male (E) and female (F) mice and weighed (n = 5). (G, H) Ratio of subcutaneous to visceral fat mass was calculated for male (G) and female (H) mice and plotted. (I, J) Ratio of visceral fat mass to total body mass was calculated and plotted. P value < 0.05 = *, ns = non-significant.