Research Paper Volume 16, Issue 9 pp 8086—8109
Constructing a novel prognostic model for triple-negative breast cancer based on genes associated with vasculogenic mimicry
Figure 7. Depletion of KCND2 and VSTM4 reduces the proliferation and migration of TNBC cells in vitro. (A) The knockdown efficiency of siRNA targeting KCND2 in BT-549 cells was verified. (B) EdU incorporation analysis and (C) CCK-8 assay revealed that the knockdown of KCND2 affected the cell proliferation in BT-549 cells. (D) The knockdown efficiency of siRNA targeting VSTM4 in MDA-MB-231 cells was verified. (E) EdU incorporation analysis and (F) CCK-8 assay revealed that the knockdown of VSTM4 impacts cell proliferation in MDA-MB-231 cells. (G) The wound healing assay demonstrated that the knockdown of KCND2 affected the migration of BT-549 cells. (H) Wound healing assay results showed that the knockdown of VSTM4 impacted the migration of MDA-MB-231 cells. *P < 0.05, **P < 0.01, ***P < 0.001.