Research Paper Volume 16, Issue 9 pp 8070—8085
KLF4 inhibited the senescence-associated secretory phenotype in ox-LDL-treated endothelial cells via PDGFRA/NAMPT/mitochondrial ROS
Figure 5. KLF4/PDGFRA regulated NAMPT/MitoROS expression. (A) Western blotting analysis of NAMPT protein expression in cultured HUVECs (n=5). (B) Immunohistochemical detection of NAMPT protein expression in the intima of high-fat diet-fed mice or EC KLF4-/- mice. Scale bar, 200 μm. Representative images (n=5) are shown. Red arrow, NAMPT-positive endothelial cells. (C) Immunofluorescence detection of MitoROS in cultured HUVECs. Scale bar, 20 μm. Representative images (n=5) are shown. Red, ROS. Green, Mitochondria. Yellow, MitoROS. (D) Flow cytometry analysis for MitoROS quantification in KLF4-treated HUVECs after altering NAMPT expression (n=5). *P < 0.05. (E) Flow cytometry analysis of MitoROS quantification in PDGFRA-treated HUVECs after altering NAMPT expression (n=5). *P < 0.05.