Research Paper Volume 16, Issue 9 pp 8070—8085
KLF4 inhibited the senescence-associated secretory phenotype in ox-LDL-treated endothelial cells via PDGFRA/NAMPT/mitochondrial ROS
Figure 3. KLF4 promotes PDGFRA transcription. (A) PPI network analysis in cultured HUVECs. (B) Western blotting analysis of the PDGF pathway in HUVECs. (C) The amounts of PDGFRA, PDGF-BB and PDGFRB in (B) quantified using actin as control. (n=5). *P < 0.05. (D) Seed region for the KLF4 binding site. (E) Luciferase activity analysis of the PDGF pathway in HUVECs (n=5). *P < 0.05. (F) EMSA analysis of the PDGF pathway in HUVECs (n=3). *P < 0.05. (G) Luciferase activity of mutant PDGFRA in HUVECs (n=5). *P < 0.05.