Research Paper Volume 16, Issue 9 pp 8019—8030

Figure 6. AURKB inhibition with AZD1152 decreased CCNE1 expression and retarded CRC progression. (A) The viability of CRC cells (HCT116, SW620, SW480, HT29, LOVO) treated with increasing concentrations of AZD1152 was evaluated using CCK-8 assay. (B) The IC₅₀ value of AZD1152 in CRC cells. (C) Western blot detected AURKB and CCNE1 protein level and pH3S10 in HCT116 and SW620 cells treated with 0, 10, 20, 50 nM AZD1152 for 48h. (D) The morphology of cells treated with AZD1152 was observed using a microscope. (E) The mRNA expression of AURKB and CCNE1 in HCT116 and SW620 cells treated with 0, 10, 20, 50 nM AZD1152 for 48h. (F, G) HCT116 and SW620 cells treated with 0, 10, 20, 50 nM AZD1152 were subjected to detect colony formation (F), cell cycle (G). (H) The apoptosis of HCT116 and SW620 cells treated with 20 nM was analyzed using flow cytometry. (H) *p < 0.05, **p < 0.01.