Research Paper Volume 16, Issue 9 pp 8019—8030
AURKB promotes colorectal cancer progression by triggering the phosphorylation of histone H3 at serine 10 to activate CCNE1 expression
Figure 3. AURKB targeted CCNE1 in CRC cells. (A) RT-qPCR detected the expression of CCND1, CDK2, CDK4, CDKN1A, and CCNE1 in shNC- or shAURKB-transfected HCT116 and SW620 cells. (B) Western blot assessed CCNE1 protein level in shNC- or shAURKB-transfected HCT116 and SW620 cells. (C, D) The mRNA and protein expression of AURKB and CCNE1 in oeNC- or oeAURKB-transfected HCT116 and SW620 cells. (E) The correlation between AURKB and CCNE1 expression in CRC tissues was obtained from TCGA database. (F) ChIP assay evaluated the effect of AURKB knockdown on the relative enrichment of pH3S10 in the promoter region of CCNE1. **p < 0.01, ***p < 0.001.