Research Paper Volume 16, Issue 9 pp 7979—7999

Figure 7. Xiaochaihu decoction induced the apoptosis of breast cancer cells by inhibiting the PI3K/Akt signaling pathway. (A) The cells were treated with the XCH decoction for 48 hours, and the protein expression of the PI3K/Akt signaling pathway was detected by Western blotting. (B) Cell viability was determined by CCK-8. (C, D) The cells were pretreated with a PI3K agonist (740 Y-P, 25 μg/mL) for 3 hours and then treated with or without the XCH decoction (20 μg/mL) for 48 hours. The expression levels of proteins associated with apoptosis and PI3K/Akt signaling pathway proteins were measured by Western blotting. (E) Knockdown of PI3K in breast cancer cells by lentivirus. (F) CCK-8 was used to detect the effect of the XCH decoction on breast cancer cells after PI3K knockdown. (G, H) Effect of the XCH decoction on apoptosis and PI3K pathway-related protein expression in breast cancer cells after PI3K knockdown. The data are shown as the mean ± SD of three experiments. ***P < 0.001 compared with the control group.