Research Paper Volume 16, Issue 9 pp 7870—7888
Leptin attenuates the osteogenic induction potential of BMP9 by increasing β-catenin malonylation modification via Sirt5 down-regulation
Figure 7. The effects of Sirt5 on Leptin-induced malonyl modification of β-catenin. (A) Western blot assay shows the effect of overexpressing Leptin on Sirt5. (B) Semi-quantification of the level of Sirt5. (C) Western blot assay shows the effect of knocking down Leptin on Sirt5. (D) Semi-quantification of the level of Sirt5. (E) Confocal assay shows the effect of knocking down Sirt5 on β-catenin. (F) Western blot assay shows the effect of knocking down Sirt5 on the level of lysine malonylation and β-catenin. (G, H) Semi-quantification of the level of β-catenin or malonylation. (I) IP assay shows the possible interaction between Sirt5 and β-catenin. (J) Western blot assay shows the effect of knocking down Sirt5 and/or Leptin on β-catenin and malonylation modification. (K, L) Semi-quantification of the level of β-catenin or malonylation. (“*” P < 0.05, “**” P < 0.01; “#” P < 0.05, “##” P < 0.01).