Research Paper Volume 16, Issue 9 pp 7870—7888
Leptin attenuates the osteogenic induction potential of BMP9 by increasing β-catenin malonylation modification via Sirt5 down-regulation
Figure 5. The effects of Leptin and/or β-catenin on BMP9-induced osteogenic markers and bone defect repair. (A) Western blot assay shows the effect of overexpressing Leptin and/or β-catenin on Runx2. (B) Semi-quantification of the level of Runx2. (C) Western blot assay shows the effect of knocking down Leptin and/or β-catenin on Runx2. (D) Semi-quantification of the level of Runx2. (E) ALP staining shows the effect of overexpressing Leptin and/or β-catenin effect on ALP activities. (F) ALP staining shows the effect of knocking down Leptin and/or β-catenin on ALP activities. (G) The 3D reconstruction of μ-CT scanning data shows the effect of β-catenin and/or Leptin on BMP9-induced bone repair (representative data are shown). (H) Quantitative analysis of μ-CT scanning (bone mineral density). (I) Images show the infection efficiency of AdGFP, Adβ-catenin, and Adsiβ-catenin in C3H10T1/2 cells (48 h). (J, K) Western blot assays show the effect of recombinant adenovirus on β-catenin in C3H10T1/2 cells (48 h). The scale bar is 200 μm. (“*” P < 0.05, “**” P < 0.01; “#” P < 0.05, “##” P < 0.01).