Research Paper Volume 16, Issue 9 pp 7870—7888
Leptin attenuates the osteogenic induction potential of BMP9 by increasing β-catenin malonylation modification via Sirt5 down-regulation
Figure 3. The effects of Leptin on BMP9-induced osteogenic markers in C3H10T1/2 cells. (A–D) qPCR and Western blot assay shows the effect of overexpressing Leptin on Runx2 and OPN. (E) ALP staining shows the effect of overexpressing Leptin on ALP activity. (F) Quantitative result of ALP staining. (G–J) qPCR and Western blot assay show the effect of silencing Leptin on Runx2 and OPN. (K) ALP staining shows the effect of silencing Leptin on ALP activity. (L) Quantitative results of ALP staining. The scale bar is 200 μm. (“*” P < 0.5, “**” P< 0.01; “#” P<0.05, “##” P < 0.01).