Research Paper Volume 16, Issue 9 pp 7870—7888
Leptin attenuates the osteogenic induction potential of BMP9 by increasing β-catenin malonylation modification via Sirt5 down-regulation
Figure 2. The effects of BMP9 on Leptin in C3H10T1/2 cells. (A) Endogenous Leptin levels in multipotent progenitor cells. (B) Semi-quantification of the level of Leptin. (C) qPCR assay shows the effect of BMP9 on Leptin. (D) Western blot assay shows the effect of BMP9 on Leptin. (E, F) Semi-quantification of the level of BMP9 or Leptin. (G) ELISA assay shows the effect of BMP9 on the concentration of Leptin in culture medium. (H, I) Images show the transduction of recombinant adenovirus in C3H10T1/2 cells. (J–N) Western blot and qPCR assay show the effect of recombinant adenovirus on Leptin (48 h). The scale bar is 200 μm. (“*” P<0.05, “**” P < 0.01).