Research Paper Volume 16, Issue 8 pp 7474—7486

Figure 2. X5 reduced H₂O₂-induced oxidative stress injury in PC12 cells. PC12 cells were incubated with X5 (0.625, 1.25, 2.5, 5 and 10 μM), CUR, TBHQ and NAC (10 μM) for 42 h (A) and 18 h (B), cells were incubated with H₂O₂ (600 μM) for 24 h (B). Cell viability was measured by MTT assay. X5 (1, 5 and 10 μM) at different concentrations and CUR (5 μM) were pre-incubated on PC12 cells for 18 h, followed by stimulation with H₂O₂ (800 μM) for 3 h (C, D) or 6 h (E), and ROS and MDA levels were measured according to the manufacturer’s methods. (F) X5 inhibited LDH release in a dose dependent manner. PC12 cells were exposed to different concentrations of X5 and CUR (5 μM) for 18 h, then the cells were treated with H₂O₂ (600 μM) for 24 h, and LDH release was detected according to the manufacturer’s methods. The data are expressed as mean ± SD, n ≥3. ^####p<0.0001, ^###p<0.0001, ^#p<0.05 vs DMSO, ^****p<0.0001, ^***p<0.001, ^**p<0.01, ^*p<0.05 vs H₂O₂.