Research Paper Volume 16, Issue 8 pp 7311—7330
EIF3B stabilizes PCNA by counteracting SYVN1-mediated ubiquitination to serve as a promotor in cholangiocarcinoma
Figure 4. EIF3B mediated cholangiocarcinoma progression through regulating PCNA. (A–C) Validation of the effects of EIF3B overexpression on the inhibitory effects of PCNA knockdown on cholangiocarcinoma progression, including proliferation (A), migration (B), and apoptosis (C), in REB cells. Scale bar: 50 μm for transwell assay. Magnification times: 200 ×. NC (OE+KD): Control, EIF3B+NC-shPCNA: EIF3B overexpression, shPCNA+NC-EIF3B: PCNA downregulation, EIF3B+shPCNA: EIF3B overexpression plus PCNA downregulation. (D–F) Validation of the effects of EIF3B knockdown on the promoting effects of PCNA overexpression on cholangiocarcinoma progression, including proliferation (D), migration (E), and apoptosis (F), in REB cells. Scale bar: 50 μm for transwell assay. Magnification times: 200 ×. NC (OE+KD): Control, PCNA+NC-shEIF3B: PCNA overexpression, shEIF3B+NC-EIF3B: EIF3B downregulation, PCNA+shEIF3B: PCNA overexpression plus EIF3B downregulation. (G) The expression of EIF3B, PCNA and P21 in HCCC-9810 cells transfected with shEIF3B and shCtrl was detected by western blot. (H) HCCC-9810 cells transfected with shEIF3B and shCtrl were treated with UC2288, and the levels of PCNA and P21 were detected by western blot. All the experiments were in triplicate. The data are expressed as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.