Research Paper Volume 16, Issue 8 pp 7217—7248

Figure 7. Hepatic METTL3 homozygous knockout by Alb-Cre mice (JAX) induces lipid accumulation in mouse hepatocytes. (A) Representative images of H&E (upper panel A) and Oil-Red-O (ORO) (lower panel A) staining of liver sections from control mice and METTL3^Δhep mice (JAX) at 1 month and 3 months postnatally. Scale bar = 100 μm. (B) Quantitative analysis of ORO staining-positive areas of frozen liver sections (shown in Figure 7A, lower panel A) relative to total liver section areas. ORO-positive areas were quantified for each of the five random images using ImageJ Software. (C) Heatmap (RNA-seq data deposited in NCBI GEO under the accession number GSE176113 [20]) depicts the differential expression of genes involved in cell cycle, hepatic lipid metabolism and cytochrome P450. In the cluster heatmap, class comparison and hierarchical clustering of differentially expressed genes (DEGs) involved in cell cycle, hepatic lipid metabolism and cytochrome P450 were performed between control mice and METTL3^Δhep mice (JAX). Genes with increased and reduced expressions are shown in red and blue, respectively. (D) GO analysis of up- and downregulated genes derived from RNA-seq data deposited in NCBI GEO under the accession number GSE176113 [20] related with cell cycle, hepatic lipid metabolism and cytochrome P450 in the liver of control mice and METTL3^Δhep mice (JAX).