Research Paper Volume 16, Issue 8 pp 7174—7187
Promoter hypomethylated PDZK1 acts as a tumorigenic gene in glioma by interacting with AKT1
Figure 4. PDZK1 knockdown inhibits the proliferation and invasion of glioma cells. (A, B) The effect of PDZK1 knockdown on U251 and U87 cell proliferation was assessed by the CCK-8 cell growth assay. ***P < 0.001. (C, D) The effect of PDZK1 knockdown on U251 and U87 cell proliferation was assessed by a colony formation assay. (E, F) Transwell assays were used to detect the inhibitory effect of PDZK1 knockdown on invasion in U251 and U87 cells. **P < 0.01. (G, H) Knockdown of PDZK1 inhibited DNA replication in U251 and U87 cells, as determined by EdU staining. **P < 0.01.