Research Paper Volume 16, Issue 8 pp 7174—7187

Figure 1. PDZK1 is upregulated in glioma tissues, and high PDZK1 expression is associated with poor prognosis. (A) The PDZK1 expression data in glioma tissues were obtained from TCGA datasets (GEPIA); GBM tissues have a higher PDZK1 expression level than normal tissues. (B) PDZK1 expression data were obtained from CGGA datasets; the PDZK1 expression level was associated with tumor histologic grade. (C) Real-time qPCR was used to detect the mRNA expression of PDZK1 in glioma tissues, and the expression of PDZK1 was much greater in glioma tissues than in normal brain tissues (p=0.0019). (D) Western blotting was used to detect the protein expression of PDZK1 in glioma tissues, and the protein expression of PDZK1 was much greater in glioma tissues than in normal brain tissues. (E) Immunohistochemical staining showed that PDZK1 expression is much greater in glioma tissues than in normal brain tissues, as shown in the image obtained from The Human Protein Atlas. (F, G) Glioma patients with lower PDZK1 expression had a more favorable survival time than did glioma patients with higher PDZK1 expression. The Kaplan–Meier method was used for this analysis. (F) data from CGGA; (G) data from TCGA.