Research Paper Volume 16, Issue 8 pp 6757—6772
USP15 facilitates the progression of bladder cancer by amplifying the activation of the NF-κB signaling pathway
Figure 6. USP15 regulates bladder cancer cell growth and invasion through NF-κB signaling pathway. (A) LinkedOmics was used to analyze KEGG pathways of USP15 in bladder cancer. The NF-κB signaling pathway is marked red. FDR, false discovery rate. (B) GSEA results showed that the samples with high expression of USP15 had high activation of NF-κB signal pathway. (C) Western blotting was used to detect the expression of USP15 and p-P65 proteins in J82 cells stably transfected with control shNC RNA or USP15 shRNA. (D) Western blotting was used to detect the expression of USP15 and p-P65 proteins in J82 cells transfected with Flag-USP15 and with the treatment of BAY11-7082. (E) The proliferation of J82 cells treated with His-USP15 was detected by colony formation test (**p < 0.01). Treatment with BAY11-7082 significantly reduced the proliferation of J82-His-USP15 cells (**p < 0.01). (F) Treatment with BAY11-7082 significantly reduced the migration and invasion of J82-His-USP15 cells (**p < 0.01).