Research Paper Volume 16, Issue 13 pp 10765—10783

Figure 5. CALCR mediated renal carcinoma progression is depended on CD44. CALCR overexpression and CD44 depletion in 786-O cell were confirmed by (A) qPCR and (B) western blot assays. GAPDH served as an internal control in qPCR and western blot assays. (C) Cell viability in 786-O cell transfected with indicated lentiviruses was assessed by Celigo cell count assay. (D) Cellular apoptosis in 786-O cell transfected with indicated lentiviruses was evaluated using flow cytometry. (E) Cell migration was analyzed by wound-healing assays. The representative images were selected from at least 3 independent experiments. Results were presented as mean ± SD. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.