Figure 3. CALCR is indispensable for in vivo tumor formation. (A) Tumor size was measured as shown, and the tumor growth curve was graphed based on tumor size. (B) All mice and corresponding tumor tissues in shCtrl group and shCALCR group was shown as the figure. Right panel presented the tumor. (C) CALCR protein level in tumor tissues isolated from mice in shCtrl group and shCALCR group was determined by western blot assay. (D) Representative Ki-67 staining images of tumor tissues in shCtrl group and shCALCR group. The quantitative statistical analysis for Ki67 expression was shown on the right. Scale bar represent 50 μm. Results were presented as mean ± SD. ***p < 0.001.