Research Paper Volume 16, Issue 13 pp 10765—10783

Figure 2. CALCR promotes malignant phenotypes of renal carcinoma cells. (A) Cell proliferative capacities of 786-O and ACHN cells transfected with shCtrl or shCALCR lentivirus were evaluated by Celigo cell count assay. (B) Alterations of cell apoptosis in 786-O and ACHN cells transfected with shCtrl or shCALCR lentivirus were analyzed by flow cytometry. (C, D) Cell migratory abilities of 786-O and ACHN cells transfected with shCtrl or shCALCR lentivirus were assessed by (C) transwell assay and (D) wound-healing assay. (E) The effect of CALCR on cell cycle distribution was analyzed by flow cytometry. The representative images were selected from at least 3 independent experiments. Results were presented as mean ± SD. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.