Research Paper Volume 16, Issue 3 pp 2005—2025

Figure 4. EC-specific VDR knockdown decreases the number of Dl⁺ cells. (A) EC-specific knockdown of VDR decreases the number of Delta⁺ cells. Flies carrying the Myo^ts>GFP or Myo^ts>GFP+VDRRi genotypes were incubated at 29° C for 1 week. The whole guts of flies were dissected, fixed, and labeled with anti-Dl (red) and anti-GFP (green) antibodies and DAPI (blue). The original magnification is 400×. (B) Frequency of Dl⁺ cells per Myo^- small cell. Three-day-old females were shifted to 29° C for 1 week, and their dissected guts were fixed and immunostained with anti-Dl (red) and anti-GFP (green) antibodies and DAPI (blue). The number of Dl⁺ cells in the Myo^- small cells was determined in these guts. Data (mean ± standard error) in Myo^ts>GFP or Myo^ts>GFP+VDRRi flies were collated from 882 and 1192 Myo^- cells of 16 and 15 guts, respectively. P-values were determined using Student’s t-test. P < 0.0001 compared with Myo^ts>GFP flies.